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KMID : 0362919900080020137
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Journal of the Korean Society for Therapeutic Radiology and Oncology
1990 Volume.8 No. 2 p.137 ~ p.144
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Quantitative Analysis of DNA Single-strand Breaks in EL 4 cells and Mouse Spleen Lymphocytes after Irradiation
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Yoo Seong-Yul
Cho Chul-Koo
Koh Kyung-Hwan
Park Woo-Yoon
Park Young-Hwan
Kim Sung-Ho
Kim Tae-Hwan
Chung In-Yong
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Abstract
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The filter elution technique was used to assay Co-60 g ray-induced DNA single-strand breaks (SSB) in EL 4 mouse leukemia cell and spleen lymphocyte.
The lymphocytes were stimulated with lipopolysaccharide (LPS, 20 ㎍/ml) to label [3H] thymidine. EL 4 cells and lymphocytes in suspension were exposed at 0℃ to Gy, 1 Gy, 5 Gy, 10 Gy of Co-60 radiation and elution procedure was performed at PH 12.1.
The number of DNA single-strand breaks increased with increasing doses of g rays. The strand scission factor (SSF) was estimated in each experiment (eluted volume 21 ml. The slope for EL 4 cells was 0.01301±0.00096 Gy-1(n=5) and the slope for lymphocytes was 0.01097±0.00091 Gy-1(n=5). The slopes were significantly different (P<0.005). Thus EL 4 cells were more sensitive to induction of DNA SSB by ionizing radiation than lymphocytes.
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KEYWORD
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Alkaline elution, EL 4, Lymphocytes, Single strand breaks, Fi
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